Research Report

Abiotic Stress Response in Yeast of A Type 1 Alternative Oxidase (LpAOX1) from Lilium pumilum  

Chang Xu , Shiya Chen , Hao He , Shumei Jin
Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
Author    Correspondence author
Molecular Microbiology Research, 2018, Vol. 8, No. 2   doi: 10.5376/mmr.2018.08.0002
Received: 13 Jul., 2018    Accepted: 24 Aug., 2018    Published: 21 Sep., 2018
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Xu C., Chen S.Y., He H., and Jin S.M., 2018, Abiotic stress response in yeast of a type 1 alternative oxidase (LpAOX1) from Lilium pumilum, Molecular Microbiology Research, 8(2): 23-29 (doi: 10.5376/mmr.2018.08.0002)

Abstract

We cloned an LpAOX1 gene from Lilium puminlum, explored this gene expression in yeast under salts (NaCl, Na2CO3, NaHCO3), pH value or oxidation (H2O2) stress, the transgenic yeast growth better than untransgenic yeast in presence of stresses. The result reveal that LpAOX1 gene has effect on the yeast tolerance to the stresses and contribute to the elucidation of the LpAOX1 molecular mechanisms involved in the stress response pathway. LpAOX1 expression in the yeast control harmful ROS contents generation, alleviate the ROS induced oxidative stress and the resistance of transgenic yeast to salt stress was enhanced.

Keywords
Alternative oxidase gene; Lilium pumilum; Yeast; Stress
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