An Ac/Ds-mediated Insertional Mutagenesis System for Functional Genomics in Sorghum
Amrish H. Antre
Ramesh S. Bhat
Department of Biotechnology, University of Agricultural Sciences, Dharwad, 580005, India5, India
Molecular Plant Breeding, 2013, Vol. 4, No. 27 doi: 10.5376/mpb.2013.04.0027
Received: 31 Jul., 2013 Accepted: 14 Aug., 2013 Published: 16 Aug., 2013
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Preferred citation for this article:
Antre et al., 2013, An Ac/Ds-mediated Insertional Mutagenesis System for Functional Genomics in Sorghum, Molecular Plant Breeding, Vol.4, No.27 220-227 (doi: 10.5376/mpb.2013.04.0027)
Transgenic sorghum (cv M 35-1) plants, carrying insertional inactivation tagging Ds, were crossed with iAc plants as the source of transposase to produce mutagenic F1 plants carrying both Ds and iAc. Screening of F2 plants for the presence of Ds (Ds+), transposed (TDs+) and absence of iAc (iAc-) could identify eight Ds tagged insertional inactivation tagged mutants. Ds transpositions unlinked to the original site of launch pad (T-DNA+Ds) integration were more frequent (57%) than linked transpositions. Analysis of flanking sequence tags of the Ds among the four mutants showed gene tagging in all. Preliminary phenotyping of the Ds tagged mutants (without ascertaining their zygosity) under green house conditions showed no major difference for morphological traits when compared to M 35-1. With the demonstration of both transiently expressed transposase (TET) system and hybridization of iAc and Ds plants to generate Ds tagged mutants, now it should be possible to develop more mutants for sorghum functional genomics.
Sorghum; Transposon tagging; Insertional inactivation tagging; Linked and unlinked transposition; Tagged genes