Sesame Shoot Regeneration-Using Different Combinations of Growth Regulators
Bangaremma S. Wadeyar
Gayatree G. S.
Plant Tissue Culture and Molecular Laboratory Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Raichur, India
Molecular Plant Breeding, 2013, Vol. 4, No. 33 doi: 10.5376/mpb.2013.04.0033
Received: 26 Aug., 2013 Accepted: 11 Oct., 2013 Published: 15 Oct., 2013
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Preferred citation for this article:
Bangaremma et al., Sesame Shoot Regeneration-Using Different Combinations of Growth Regulators, Molecular Plant Breeding, Vol.4, No.33 267-269 (doi: 10.5376/mpb.2013.04.0033)
Sesame (Sesamum indicum L.) an important and oldest oil seed crop known to man still, the regeneration studies remained as a bottleneck. Here an attempt was to develop shoot via tissue culture approaching using hypocotyls callus, for this five genotypes viz GLW (Gulbarga local white), Western-II (W-II), DS-I, E-8 and Tumkur local (TL). Healthy and moderately compact callus was obtained from hypocotyls segments placed in the plane MS media were used as explants. These obtained callus were placed on the media containing TDZ of concentration 25 µM and IAA (2 µM &3 µM). The maximum number of shoot regeneration was in variety DS-I with 83.3% & 75% response achieved on MS media containing TDZ 25 µM and IAA 3 µM, 2 µM respectively, followed by 58% shoot response in variety E8 and TL respectively on MS media containing TDZ 25 µM and IAA 3 µM, 2 µM respectively and the mean shoot regeneration percent was 52.35 and number of shoots pre callus were 1.59. The shoot regeneration will increase the chances of genetic improvement of sesame through biotechnological ways such as genetic transformation and in vitro cell line selections.
Sesamum indicum; Hypocotyl’s callus; High frequency shoots regeneration; TDZ