Functional Analysis of Synthetic Promoters Containing Pathogen-Responsive cis-Elements
Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, India
Molecular Plant Breeding, 2013, Vol. 4, No. 34 doi: 10.5376/mpb.2013.04.0034
Received: 25 Sep., 2013 Accepted: 15 Oct., 2013 Published: 17 Oct., 2013
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Preferred citation for this article:
Yeri et al., Functional Analysis of Synthetic Promoters Containing Pathogen-Responsive Cis-Elements, Molecular Plant Breeding, Vol.4, No. 34 270-276 (doi: 10.5376/mpb.2013.04.0034)
An attempt was made to study four synthetic promoters containing pathogen-responsive cis-elements with -46 region minimal promoter of CaMV 35S for their inducibility in tobacco upon exposure to salicylic acid (SA) and methyl jasmonate (MeJ). On 15th day of establishment, none of the untreated plants, except those carrying CaMV 35S-driven SgfpS65T showed reporter expression. Plants sprayed with 5 mM SA or MeJ showed SgfpS65T expression after 5 min, 1 hr, 2 hr, 3hr and 7 hr. GCC2X and S2X were induced by both SA and MeJ, W2X was induced by SA, but not by MeJ. However, GCC3X promoter did not show any induction. With SA, the strength of induction of W2X and GCC2X promoters as measured by ImageJ software were comparable and relatively higher than S2X. Similarly, GCC2X and S2X had similar magnitude of induction with MeJ. In general, the synthetic promoters were more strongly induced by SA compared to MeJ, however their strength was less compared to that of CaMV 35S promoter.
S.B. Yeri; R.S. Bhat; M.S. Kuruvinashetti