Genetic Diversity of the Selected 64 Potato Germplasms Revealed by AFLP Markers
1.Institute of Biotechnology of Qinghai Academy of Agriculture and Forestry, Xining, 810016, P.R. China
2.The Qinghai-Tibet Plateau Biotechnology Key Lab of Ministry of Education, Xining, 810016, P.R. China
3.Qinghai University, Xining, 810016, P.R. China
Molecular Plant Breeding, 2011, Vol. 2, No. 4 doi: 10.5376/mpb.2011.02.0004
Received: 28 Dec., 2010 Accepted: 06 Jan., 2011 Published: 25 Feb., 2011
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Preferred citation for this article:
Wang et al., 2011, Genetic diversity of the selected 64 potato germplasms revealed by AFLP marker, Molecular Plant Breeding, Vol.2 No.4 (doi: 10.5376/mpb.2011.02.0004)
In order to utilize the elite germplasms from the International Potato Center (CIP) for potato breeding program in China, we analyzed the genetic relationship of selected 64 potato cultivars based on the AFLP approach in this study. Twelve polymorphic primer combinations were screened out to be used for analyzing the genetic diversities among the tested materials. The number of bands generated by each selected AFLP primer pair reached about 42 in the ranged of from 33 to 56. Total of 505 reproducible bands were amplified by 12 primer, of which polymorphic bands were 388. The genetic distance were calculated among the tested 64 potato cultivars in the range from 0.041 237 1 to 0.391 732 6. The cluster analysis showed that domestic cultivars were assigned to be a group at the genetic coefficient of 0.82 and all tested potato cultivars were clustered together at the genetic similarity coefficient (GS) of 0.68. Shannon’s Information Index of examined materials was between 0.245 6 and 0.498 8 with an average of 0.374 5, as well as Nei’s Gene Diversity Index of examined materials varied from 0.151 0 to 0.325 6 with a mean of 0.235 9. In this research, we might have a conclusion that that genetic basis of domestic potato cultivars is quite narrow compared to the accessions of potato resources from CIP. Therefore, the CIP germplasms can be used for broadening the genetic basis of local potato cultivar in China.
Potato; Potato Germplasms; Genetic Diversity; AFLP