Deep Sequencing of the Pistil Transcriptome in the Cross Incompatible Maize  

Song Li1 , Chunyan Xu1 , Dezhou Cui1 , Huaihua Liu1 , Peng Li1 , Hua Zhang2 , Detao Li1 , Youhui Tian1 , Huabang Chen2 , Xianrong Zhao2
1. The State Key Lab of Crop Biology, College of Agriculture, Shandong Agricultural University, Tai’an, Shandong, P. R. China
2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China
Author    Correspondence author
Molecular Plant Breeding, 2014, Vol. 5, No. 10   doi: 10.5376/mpb.2014.05.0010
Received: 14 Apr., 2014    Accepted: 21 Apr., 2014    Published: 11 Jul., 2014
© 2014 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Li et al., 2014, Deep Sequencing of the Pistil Transcriptome in the Cross Incompatible Maize, Molecular Plant Breeding, 2014, Vol.5, No. 10 1-14 (doi: 10.5376/mpb.2014.05.0010)

Abstract

Plant reproductive development is dependent on successful pollen-pistil interactions. The high degree of specificity in pollen-pistil interactions and the precision of directional pollen tube growth suggest that signals are continually being exchanged between pollen tubes and cells of the pistil that line their path. However, with few exceptions, little is known about the genes that control these interactions. In this investigation, we performed genome-wide transcriptional profiling of pistil tissues of Gametophytic factor1-Strong (Ga1-S) genotype maize after pollination 5 hours(5HAP)with Ga1-S pollen and ga1 pollen respectively , in an attempt to isolate genes involved in pollen tube elongation in maize pistils, using the Solexa deep sequencing technology. The results of this study suggest that the regulation of reinforcing, modifying, or remodeling the structure of the transmitting tract and the pollen tube tip play a critical role in pollen-pistil interactions during pollen tube elongation in maize pistils, and the inhibition of these functions maybe the molecular mechanism of how Gametophytic factor1-Strong (Ga1-S) presence in style tissue completely prevents ga1 pollen to accomplish fertilization.

Keywords
Deep sequencing; Transcriptome; Cross incompatibility; Pistil; Maize
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