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Genetic Diversity Analysis of Eritrean Sorghum ( Sorghum bicolor (L.) Moench) Germplasm using SSR Markers | Tesfamichael 1,2 | Molecular Plant Breeding

Genetic Diversity Analysis of Eritrean Sorghum (Sorghum bicolor (L.) Moench) Germplasm using SSR Markers  

Tesfamichael Abraha Tesfamichael1,2 , S. M. Githiri2 , R. W. Kasili2 , R.A. Skilton3 , M. Solomon3 , A.B. Nyende2
1. Hamelmalo Agricultural College (HAC), P.O.Box 397, Keren, Eritrea
2. Jomo Kenyatta University of Agriculture and Technology (JKUAT, P.O.Box 62000-0020, Nairobi, Kenya
3. Biosciences eastern and central Africa- International Livestock Research Institute (BecA-ILRI) Hub, P.O Box 30709 Nairobi 00100 Kenya
Author    Correspondence author
Molecular Plant Breeding, 2014, Vol. 5, No. 13   doi: 10.5376/mpb.2014.05.0013
Received: 20 Jun., 2014    Accepted: 18 Jul., 2014    Published: 23 Oct., 2014
© 2014 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Tesfamichael et al., Genetic Diversity Analysis of Eritrean Sorghum (Sorghum bicolor (L.) Moench) Germplasm using SSR Markers, Molecular Plant Breeding, 2014, Vol.5, No. 13 1-12 (doi: 10.5376/mpb.2014.05.0013)

Abstract

Eritrea is considered a center of origin for sorghum, the main cereal crop in terms of area under cultivation and production in the country. There have been very little genetic diversity studies done on the Eritrean sorghum before. To improve this crop, the knowledge of genetic diversity estimation is needed on the available germplasm. The aim of this study was therefore to asses the extent of genetic diversity within and among 98 sorghum genotypes collected from Eritrea alongside 42 regional reference accessions from the International Crop Research Institute for Semi-Arid Tropics (ICRISAT) using a set of 29 Simple Sequence Repeat (SSR) markers. An average of 4.8 alleles per marker was recorded. The mean polymorphism information content (PIC) value for the SSR loci was 0.52. The Analysis of Molecular Variation (AMOVA) revealed that 12% of the variation resulted from the difference among populations, 31% within individual populations and 57% among individual accessions of the sub populations. Neighbor joining phylogeny tree based on genetic similarity coefficient revealed three distinct groups of clustering with the Eritrean populations further sub clustered into three groups. The Eritrean sorghum accessions from Gash Barka and South regions and South Sudan accessions recorded the highest private alleles. The results of Principal Coordinate Analysis (PCoA) also classified the sorghum accessions into three major groups. Genetic distance matrix revealed that the Eritrean accessions are more related to each other compared to the regional accessions. The existence of higher level of allelic richness, close genetic distance and an isolated clustering of the Eritrean population indicates that the accessions have not been introgressed with foreign genes and are valuable resource for future breeding programs of this crop.

Keywords
Sorghum; Eritrea; SSR markers; Genetic diversity; Germplasm; Analysis of molecular variance
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