Integration and Expression Stability of Transgenes in Hybriding Transmission of Transgenic Rice Plants Produced by Particle Bombardment
1 College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou, 310035, China;
2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China;
3 College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310018, China
Molecular Plant Breeding, 2011, Vol. 2, No. 8 doi: 10.5376/mpb.2011.02.0008
Received: 26 Apr., 2011 Accepted: 03 May, 2011 Published: 20 Jun., 2011
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Preferred citation for this article:
Zhao et al., 2011, Integration and Expression Stability of Transgenes in Hybriding Transmission of Transgenic Rice Plants Produced by Particle Bombardment Vol.2 No.12 (doi: 10.5376/mpb.2011.02.0008)
Four transgenic rice lines TR 5, TR 6, Ming B and Jingyin 119 obtained via particle bombardment were used as transgene donors to create hybrids. The integration and expression stability of exotic bar and cecropin B gene in conventional hybriding transmission were investigated by Southern and Northern blotting analyses. The selection marker bar gene was transferred to all hybrids under selection of Basta herbicide. Loss or gain of small hybridization bands (no more than 2.0 kb) of bar gene occurred in some hybrids, but the difference in integration sites of bar gene copies did not influence their stable expression. The non-selection gene cecropin B was stably transferred from the four transgene donors to their resulting hybrids, but expression level was very different. Silencing of cecropin B gene occurred in some hybrids from TR 5, TR 6 and Ming B. In the transgene donor Jingyin 119 and all its resulting hybrids, cecropin B and bar gene were stably expressed. We concluded that the stability of transgene during crossbreeding transmission is mainly determined by the primary transgenic donors and may be affected by recombination.
Transgene; Heredity and expression; Hybriding transmission; Particle bombardment; Rice