Research Article

Study of Dichlorophenoxyacetic Acid and 6-Benzylaminopurine Effects on Callus Development in Cucurbita moschata  

Saba Riaz1 , Athar Hussian Shah1 , Saif-ul- Malook2 , Qurban Ali3
1 Department of Botany, GC University Lahore, Pakistan
2 Kunming Institute of Botany, University of Chinese Academy of Sciences, China
3 Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
Author    Correspondence author
Molecular Plant Breeding, 2016, Vol. 7, No. 3   doi: 10.5376/mpb.2016.07.0003
Received: 07 Nov., 2015    Accepted: 22 Dec., 2015    Published: 02 Jan., 2016
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Saba Riaz, Athar Hussian Shah, Saif-ul-Malook, and Qurban Ali, 2016, Study of Dichlorophenoxyacetic acid and 6-Benzylaminopurine effects on callus development in Cucurbita moschata, Molecular Plant Breeding, 7(03): 1-12 (doi: 10.5376/mpb.2016.07.0003)

Abstract

Cucurbita moschata is an important and popular vegetable, used throughout the world due to its taste, nutritional and medicinal values. Plant growth hormones play an important role in the growth and development of crop plants. Callus development of Cucurbita moschata was accessed produced from cotyledon, leaf, hypocotyls and root under the application of auxins dichlorophenoxyacetic acid (2,4-D) and cytokinins 6-Benzylaminopurine (BAP) with different concentrations. It was found that 1.5mg/L BAP+1.5mg/L 2,4-D and 0.5mg/L BAP+1.5mg/L 2,4-D combination was best for callus development from cotyledon, as both gave a callus index of 300 but the time for callusing was 9 and 10 days respectively vs. 2,4-D and BAP (3 mg/L) for callus induction by leaves, 2mg/L BAP+1.5mg/L 2,4-D for callus induction from hypocotyl and from root the combination of 1.5mg/L BAP +3mg/L 2,4-D produced the best results. It was suggested form the results that the combination of optimum concentrations (1.5-3mg/L) of BAP and 2, 4-D may be useful for developing higher callus mass in short time.

Keywords
Cucurbita moschata; Callus; Dichlorophenoxyacetic acid; 6-Benzylaminopurine; Plant growth regulators
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