Research Ariticle

Development of Source Independent Micropropagation System in Dalbergia sissoo Roxb, as a basis for Germplasm Conservation and Disease Free Plants Production  

Shan-e-Ali Zaidi1 , Siddra Ijaz1 , Azeem Iqba Khan1,2 , Iqrar Ahmad Rana1
1 Centre of Agricultural Biochemistry and Biotechnology(CABB),University of Agriculture Faisalabad, Pakistan
2 Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
Author    Correspondence author
Molecular Plant Breeding, 2016, Vol. 7, No. 15   doi: 10.5376/mpb.2016.07.0015
Received: 10 Dec., 2015    Accepted: 19 Feb., 2016    Published: 19 Feb., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zaidi S.A., Ijaz S., Iqba A., Khan, and Rana I.A., 2016, Development of Source Independent Micropropagation System in Dalbergia sissoo Roxb, as a Basis for Germplasm Conservation and Disease Free Plants Production, Molecular Plant Breeding,7(10):1-12 (doi: 10.5376/mpb.2016.07.0015)

Abstract

Plant micropropagation has becoming an imperative strategy at commercial level that provides large number of plants in less time and space. In woody trees, micropropagation is more tedious and cumbersome process than other crop plants. Less success rate in tree micropropagation has been reported in literature. Therefore in this study, expedite, reproducible and source independent micropropagation protocol was developed in Dalbergia sissoo (Shisham) that is an economically important timer yielding tree with medicinal values. For this purpose, eight micropropagation media were studied with various combination of BAP (benzylaminopurine) and IAA (indole acetic acid). Auxially and epicormic shoots of Dalbergia sissoo having at least one nodal bud were used as explants. We studied the impact of these media combination and explant types on micropropagation. We found MPMS5 is the best medium combination that gave maximum number of shoots (2.78 shoots per explant) followed by MPMS7. This selected media combination can be used for Dalbergia sissoo micropropagation as a routine work. Similarly rooting media combinations were also studied for the best root induction in micropropagated shoots of Dalbergia sissoo but little success had been achieved.

Keywords
Dalbergia sissoo; Micropropagation; Shisham; Tissue culture; Woody tree
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