Research Report

Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses to Cold Stress in Two Contrasting Medicago ciliaris Populations  

Nourredine Yahia , Asma Bouira , Chahrazed Cheriet , Fatima Zohra Fyad-Lameche
Departement de Biologie, Faculte des Sciences de la Nature et de la Vie, Universite Oran1 (Ahmed Ben Bella), BP.16 Es-senia 31000 Oran 1, Algeria
Author    Correspondence author
Molecular Plant Breeding, 2016, Vol. 7, No. 17   doi: 10.5376/mpb.2016.07.0017
Received: 17 Feb., 2016    Accepted: 02 Apr., 2016    Published: 19 Apr., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Nourredine Y., Asma B., Chahrazed C., and Fatima Z.F.L., 2016, Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses to Cold Stress in Two Contrasting Medicago ciliaris Populations,Molecular Plant Breeding, 7(17): 1-7(doi: 10.5376/mpb.2016.07.0017)

Abstract

Effect of cold on antioxidant responses in aerial (shoots and leaves) and roots tissues of two contrasting accessions of M. ciliaris Krockers (Cil 126, tolerant and Cil 123, sensible) were investigated. Ten-day-old grown seedlings were subjected at different periods of cold acclimation (4°C) 2, 4, and 6 days. Peroxidases (POD) (EC 1.11.1.7) activities and isoenzymes expression of plantlets treated and control (23°C) of the aerial (shoot+leaves) and root vegetative tissues were carried. Global peroxidase activity under low temperature stress was higher in tolerant ecotype than sensible one. The same trend was also observed at expressed isoenzymes. On the other hand, whether in tolerant or sensitive, activity and isoenzymes peroxidase was more pronounced at the roots than in aerials tissues system.

Keywords
Peroxidase activity; Isoenzymes; Cold stress; M. ciliaris; PAGE electrophoresis
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