Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses to Cold Stress in Two Contrasting Medicago ciliaris Populations
Fatima Zohra Fyad-Lameche
Departement de Biologie, Faculte des Sciences de la Nature et de la Vie, Universite Oran1 (Ahmed Ben Bella), BP.16 Es-senia 31000 Oran 1, Algeria
Molecular Plant Breeding, 2016, Vol. 7, No. 17 doi: 10.5376/mpb.2016.07.0017
Received: 17 Feb., 2016 Accepted: 02 Apr., 2016 Published: 19 Apr., 2016
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Preferred citation for this article:
Nourredine Y., Asma B., Chahrazed C., and Fatima Z.F.L., 2016, Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses to Cold Stress in Two Contrasting Medicago ciliaris Populations,Molecular Plant Breeding, 7(17): 1-7(doi: 10.5376/mpb.2016.07.0017)
Effect of cold on antioxidant responses in aerial (shoots and leaves) and roots tissues of two contrasting accessions of M. ciliaris Krockers (Cil 126, tolerant and Cil 123, sensible) were investigated. Ten-day-old grown seedlings were subjected at different periods of cold acclimation (4°C) 2, 4, and 6 days. Peroxidases (POD) (EC 126.96.36.199) activities and isoenzymes expression of plantlets treated and control (23°C) of the aerial (shoot+leaves) and root vegetative tissues were carried. Global peroxidase activity under low temperature stress was higher in tolerant ecotype than sensible one. The same trend was also observed at expressed isoenzymes. On the other hand, whether in tolerant or sensitive, activity and isoenzymes peroxidase was more pronounced at the roots than in aerials tissues system.
Peroxidase activity; Isoenzymes; Cold stress; M. ciliaris; PAGE electrophoresis