Overexpression of Arabidopsis CBF1 Gene in Transgenic Furenzhi banana (Musa spp. AA group) Improves Resistance to Low Temperature
Fruit Tree Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Biology and Genetic Resource Utilization of Fruit Trees in South Subtropics, the Ministry of Agriculture, Guangzhou, 510640, China
Molecular Plant Breeding, 2016, Vol. 7, No. 34
Received: 05 Sep., 2016 Accepted: 26 Oct., 2016 Published: 16 Dec., 2016
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Cold is an environmental factor that limits the growing season of banana and adversely affects fruit quality and productivity. C-repeat/dehydration responsive element binding factor 1 (CBF1) play important roles in the responses of plants to low-temperature and cold. To test the function and potential use of cold-induced factor CBF1 in improving the chill tolerance of banana, AtCBF1 driven by a cauliflower mosaic virus 35S promoter was introduced into banana plants by Agrobacterium-mediated transformation of embryogenic cell suspensions (ECSs) of banana cultivar Furenzhi (AA). The presence of AtCBF1 transgenes in regenerated plants was confirmed by polymerase chain reaction (PCR) and Southern blots. ß-glucoronidase (GUS) histochemical assays reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR analysis demonstrated that foreign genes were stably expressed in regenerated plants. Transgenic banana showed growth retardation, thicker leaves and higher chlorophyll content than non-transformed plants. While relative electrolyte leakage (REL) and malondialdehyde (MDA) content were significantly lower in transgenic banana plants than in non-transformed plants under low temperature stress. An obviously higher cold tolerance was observed among the transgenic plants in the cold detection. These results suggest that over-expression of AtCBF1 in transgenic banana plants plays an important role in improving tolerance to low-temperature.
Banana; AtCBF1 gene; Transgenic; Cold resistance
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