Generation and Analysis of ESTs in V. amurensis Grapevine Pericarp at Veraison  

Xiangnan Ji1* , Bo Li3* , Wen Zhang2 , Chengjun Yang3 , Jun Wang1
1. Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, P.R. China
2. College of Agriculture & Biotechnology, China Agricultural University, Beijing, 100193, P.R. China
3. College of Forestry, Northeast Forestry University, Harbin, 150040, P.R. China
* These authors contributed equally to this study
Author    Correspondence author
Molecular Plant Breeding, 2012, Vol. 3, No. 3   doi: 10.5376/mpb.2012.03.0003
Received: 22 Feb., 2012    Accepted: 28 Mar., 2012    Published: 01 Apr., 2012
© 2012 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Ji et al., 2012, Genetic Analysis of ESTs Generated from Pericarp of Wine Grape (V. amurensis) at Veraison, Molecular Plant Breeding, Vol.3, No.3 26-36 (doi: 10.5376/mpb.2012.03.0003)

Abstract

In this study, we constructed the cDNA library of grape berry pericarp at veraison from wine grape ‘Shuang Feng’ (V. amurensis), and generated total 974 ESTs, representing the independent cDNA in length of around 392,672 bp, of which 710 unigenes were clustered into 97 contigs and 613 singletons based on quality of EST sequence and optimum assembly. 64.65% clones exhibited high homology to previously deposited nucleotides and/or polypeptide sequences, while the proportion of ESTs with no any significant homology in database reached 25.63%. Analysis of the unigenes resulted in the assignment of 9 specific functional classes in this research. Putative functions were identified for about 74.13% of the unigenes. By assigning them to specific function classes, we were able to highlight characteristics among different ripening phases. GO classification was performed to define the relationship among genes expressing in pericarp of wine grape berry, which were involved in three aspects including cell component, biological process and molecular function. Expression analysis of these ESTs were used to characterize potential roles of novel genes with respect to berry ripening and composition of wine grape.

Keywords
Wine grape (Vitis amurensis); cDNA library; Unigene annotation; Gene ontology; EST sequencing
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