Mapping of Nuclear Male-Sterile Gene ms14 Using SSR Markers in Cotton
Hainan Tropical Ocean University, Sanya, Hainan, 572022, China
Molecular Plant Breeding, 2016, Vol. 7, No. 35 doi: 10.5376/mpb.2016.07.0035
Received: 25 Oct., 2016 Accepted: 14 Dec., 2016 Published: 23 Dec., 2016
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Preferred citation for this article:
Wang P.Z., Bian L., and Cao C., 2016, Mapping of nuclear male-sterile gene ms14 using SSR markers in cotton, Molecular Plant Breeding, 7(35): 1-5 (doi: 10.5376/mpb.2016.07.0035)
Nuclear male sterility (NMS) is a very important character for cotton breeding and genetics programs. Identification of linked molecular markers with NMS will greatly facilitate breeding for this trait. Among all of the Nuclear male-sterile genes, only Dong-A (ms14) was utilized successfully in hybrid production of cotton. Dong-A (ms14), a recessive NMS line developed from spontaneous mutation in upland cotton, has been applied most widely and played an increasing role in hybrid cultivar development in China. An interspecific F2 population comprised of 180 individual plants was developed by crossing a cultivar of Kang A (Dong-A derived Lines, Gossypium hirsutum L.) to a cultivar of 601588 (G. barbadense L.). Basing on this population the ms14 gene was mapped on chromosome 2 and closely linkaged with BNL3971 marker within a genetic distance of 16.7 cM. The BNL3971 marker could be used for the marker assisted selection in breeding a new cultivar line, and provide the information for closely location and further gene isolation by map based cloning.
Cotton; Molecular mapping; Nuclear male-sterile genes; ms14