Pathogen Identification of Clubroot Disease in Chinese Cabbage from Yuanyang County, Henan Province
Yang Li 1,2*
1 College of Life Science, Zhengzhou University, Zhengzhou, Henan, China
2 Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China
* These authors contributed equally to this work
Molecular Plant Breeding, 2017, Vol. 8, No. 5 doi: 10.5376/mpb.2017.08.0005
Received: 28 Feb., 2017 Accepted: 13 Jun., 2017 Published: 28 Jul., 2017
© 2017 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License
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Preferred citation for this article:
Li Y., Yuan Y.X., Zhao Y.Y., Wei X.C., Yao Q.J., Jiang W.S., Wang Z.Y., Yang S.J., Zhang X.W., and Tian B.M., 2017, Pathogen identification of clubroot disease in Chinese cabbage from Yuanyang county, Henan province, Molecular Plant Breeding, 8(5): 45-51 (doi: 10.5376/mpb.2017.08.0005)
To clarify the causal agent of clubroot disease in Chinese cabbage from Yuanyang experimental base, the isolate YY was collected from the clubroot for further research. After morphological, cytological observation and pathogenicity identification, the pathogen was preliminary inferred as Plasmodiophora brassicae. In order to quickly detect the pathogen, specific primers were designed according to D85819 and Pro1 gene, respectively, and used to amplify the expected fragment from the template DNA of target pathogen. After PCR and sequence analysis, they showed 99% and 93% identity with the D85819 and Pro1 gene sequences of P. brassicae from NCBI respectively. These results indicated that the pathogen was finally confirmed as P. brassicae, which were coincident with preliminary speculation. Moreover, Pro1-specific marker developed in this study can be applied to quick and accurate detection of P. brassicae isolate, which appears a useful assay method for detecting P. brassicae resting spores and improves efforts to halt the dissemination and better control this pathogen.
Yuanyang County; Clubroot disease; Chinese cabbage; Pathogen indentification