Genetic Diversity Analysis of Capsicum Genus by SSR Markers
1 Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China
2 College of Life Science, Zhengzhou University, Zhengzhou, 450001, China
3 Universite Paris XII Val de Marne, 61, avenue du general de gaulle 94010 creteil, France
Molecular Plant Breeding, 2017, Vol. 8, No. 8 doi: 10.5376/mpb.2017.08.008
Received: 27 Sep., 2017 Accepted: 24 Nov., 2017 Published: 04 Dec., 2017
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Preferred citation for this article:
Meng C.Y., Wei X.C., Zhao Y.Y., Yuan Y.X., Yang S.J., Wang Z.Y., Zhang X.W., Sun J.W., Yang L., Yao Q.J., and Zhang Q., 2017, Genetic diversity analysis of Capsicum genus by SSR markers, Molecular Plant Breeding, 8(8): 70-78 (doi: 10.5376/mpb.2017.08.0008)
The genetic diversity of pepper resources is rich and the potential of breeding is great. Therefore, the objectives of the study were to determine the genetic diversity and population structure of 32 accessions of Capsicum germplasm resources and contribute to breeding of pepper. In this study, the genetic diversity of different species of pepper germplasm was studied from the molecular level, which provided reference for the collection, research and rational utilization of pepper germplasm resources. 80 pairs of SSR primers were designed based on the whole genome coding region sequence of pepper. The 32 accessions of Capsicum germplasm resources of 12 species (subspecies) with different geographical origin and different traits were selected to screen 80 pairs of primers, which was to obtain clear bands, good stability of 40 SSR polymorphic primers. DPS, MEGA7 and POPGENE32 software were used to analyze the genetic diversity of 32 pepper germplasm resources. The results showed that 40 pairs of primers amplified 122 polymorphic bands, with an average of 3.05 loci amplified by each pair of primers, which showed that the SSR primers had high practicability in the genetic analysis of pepper. The mean value of effective allele number (Ne), observed heterozygosity (Ho), expected heterozygosity (He), shannon-weaver index (I), polymorphism information content (PIC) were showed that pepper genetic information is rich. Based on cluster analysis of UPGMA method and principal component analysis were basically consistent with the source of Capsicum were divided into 10 clusters.
Capsicum Genus; SSR; Genetic diversity; Cluster analysis