Research Report

Light and Dark Apporaches in Agrobacterium-Mediated Transformation in 84K Poplar  

Li-Na Wang , Jiaqi Ding , Wenshu Mao
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China
Author    Correspondence author
Molecular Plant Breeding, 2017, Vol. 8, No. 9   doi: 10.5376/mpb.2017.08.0009
Received: 27 Sep., 2017    Accepted: 24 Oct., 2017    Published: 04 Nov., 2017
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang L.N., Ding J.Q., and Mao W.S., 2017, Light and dark approaches in agrobacterium-mediated transformation in 84K Poplar,Molecular Plant Breeding, 8(9): 79-84 (doi: 10.5376/mpb.2017.08.0009)

Abstract

This paper describes two by A. tumefaciens to introduce target genes into Populus alba X Populus glandulosa and comparatively analyzed two transgenetic systems. We summarized the advantages and disadvantages of the two methods. However, the dark-induced method had higher transformation efficiency and light-induced method has shorter period. So, a combination of the two methods can help maximize on efficiency and high levels of gene expression in transgenic plants. This study provides a useful foundation for further research toward the development of 84K Poplar.

Keywords
84K Poplar; Callus; Direct differentiation; Genetic transformation; A. tumqfaciens
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