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Molecular Cloning and Expression Analysis of C Function Gene CjPLE in Double Flower Varieties of Camellia japonica | Yin | Molecular Plant Breeding

Research Report

Molecular Cloning and Expression Analysis of C Function Gene CjPLE in Double Flower Varieties of Camellia japonica  

Hengfu Yin , Tao Lyu , Jiyuan Li , Xinlei Li , Zhengqi Fan , Wen Yang , Sui Ni
1 Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, 311400, China
2 Ningbo University, Ningbo, 315211, China
Author    Correspondence author
Molecular Plant Breeding, 2019, Vol. 10, No. 3   doi: 10.5376/mpb.2019.10.0003
Received: 11 Nov., 2018    Accepted: 07 Dec., 2018    Published: 07 Feb., 2019
© 2019 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

The C function gene in the classic ABC model is the important factor that determines the stamen and pistil organs in the floral axis, and is also the regulatory gene of floral meristem termination differentiation. A 901 bp cDNA sequence of C function gene was cloned from flower bud of Camellia japonica, named CjPLE (MF278983), which contained an opening reading frame of 780 bp. The “Genome Walking” experiment showed that it contained two introns. Through phylogenetic analysis, we found that it formed a PLE subclass with TAG1 of Solanum lycopersicum and PLE of Antirrhinum majus. The results of real-time PCR showed that CjPLE gene had high expression in the carpel of wild Camellia japonica. In peony type double-flower varieties ‘Hongluzhen’ and ‘Zhuangyuanhong’, the highest expression of CjPLE gene was in stamens, while in ‘Rongqiu’, the expression of CjPLE gene in inner petals and stamens were both high. The above results indicate that CjPLE is a homologous gene of the PLE branch in which belongs to C function gene in Camellia japonica, and might play a role in regulating the development of floral organs.

Keywords
Camellia japonica; CjPLE gene; Real-time PCR; Gene structure; Phylogenetic analysis
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