Research Report

The CDS Variance Analysis of OsBADH2 Gene and Aroma Appraisal of 7 Guizhou HE (Oryza sativa)  

Lili Wang , Qiang Peng , Jiali Li , Dashuang Zhang , Jianqiang Wu , Xue Jiang , Susong Zhu
Guizhou Rice Research Institute, Guizhou Academy of Agricultural Science, Guiyang, 550006, China
Author    Correspondence author
Molecular Plant Breeding, 2018, Vol. 9, No. 12   doi: 10.5376/mpb.2018.09.0012
Received: 22 Nov., 2018    Accepted: 10 Dec., 2018    Published: 30 Dec., 2018
© 2018 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2018, 13: 4138-4142) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang L.L., Peng Q., Li J.L., Zhang D.S., Wu J.Q., Jiang X., and Zhu S.S., 2018, The CDS variance analysis of OsBADH2 gene and aroma appraisal of 7 Guizhou HE (Oryza sativa), Molecular Plant Breeding, 9(12): 87-91 (doi: 10.5376/mpb.2018.09.0012)

 

 

Abstract

Dysfunction of OsBADH2 gene in rice is the internal cause of rice to have fragrance. This study applied physicochemical methods to identify the fragrance traits of 7 Guizhou HE (Oryza sativa) and 2 control varieties. It was found that RongHe and 2 control varieties were fragrant rice, and the other 6 varieties were all non-fragrant rice. To analyze the internal relationship between fragrant production in Guizhou HE and OsBADH2 gene, the study obtained the CDS sequences of OsBADH2 gene in 9 tested materials, and conducted alignment analysis of these sequences by Megalign software through TA-cloning, sequencing analysis and sequence assembly. The results showed that the OsBADH2 gene CDS of 4 Guizhou HE (BaixiangHe 2, BanpoHe, LipingHe 1 and LipingHe 2) were normal. The CDS differences of RongHe and 2 control varieties were both 8 bp deletion and 3 SNPs in exon 7, and 2 new CDS differences were found among which BaixiangHe 1 was 1 bp deletion and 1 SNP, and BianHe was 2 bp deletion and 2 SNPs. These results might provide the theoretical basis for variety protection and breeding improvement of Guizhou HE (Oryza sativa).

Keywords
Oryza sativa L.; Fragrance; OsBADH2; TA-cloning; Sequence alignment
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