hacker tools
xnxxfix.com
şehirler arası nakliyat istanbul şehirler arası nakliyat şehirler arası nakliyat fiyatları

hack forum hack forum alfa shell recovery shell shell recovery shell Mirror Zone smm bayi smm ana bayi wso shell hacklink hacklink paketleri hacklink nedir hacklink panel

cami halısı cami halıları cami halısı cami halıları cami halısı cami halıları Cami Halısı Cami Halıları Cami Halısı Turkuaz Cami Halısı Cami Süpürgesi

Evden Eve Nakliyat istanbul Evden Eve Nakliyat istanbul Evden Eve Nakliyat Evden Eve Nakliyat mng Evden Eve Nakliyat Evden Eve Nakliyat Evden Eve Nakliyat istanbul Evden Eve Nakliyat Evden Eve Nakliyat istanbul Nakliyat evden eve nakliyat istanbul evden eve nakliyat istanbul ofis taşıma ofis taşımacılık ofis taşımacılığı istanbul ofis taşımacılığı taşıma yurtiçi evden eve nakliyat evden eve nakliyat yurtiçi evden eve nakliyat yurtiçi nakliyat içel Evden Eve Nakliyat Mersin Evden Eve Nakliyat

çiğköfte çiğköfte bayiliği çiğköfte bayilik promosyon promosyon ürünleri promosyon şapka prefabrik prefabrik evler
High-throughput Identification and Marker Development of Perfect SSR for Cultivated Genus of Passion Fruit ( Passiflora edulis ) | Wu 1 | Molecular Plant Breeding

Research Report

High-throughput Identification and Marker Development of Perfect SSR for Cultivated Genus of Passion Fruit (Passiflora edulis)  

yanyan Wu1 , Qinglan Tian1 , Jieyun Liu1 , Yongcai Huang1 , Weihua Huang1 , Xiuzhong Xia2 , Xinghai Yang2 , Haifei Mou1
1 Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China
2 Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China
Author    Correspondence author
Molecular Plant Breeding, 2018, Vol. 9, No. 13   doi: 10.5376/mpb.2018.09.0013
Received: 05 Nov., 2018    Accepted: 07 Dec., 2018    Published: 29 Dec., 2018
© 2018 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2018, 20: 6738-6743) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wu Y.Y., Tian Q.L., Liu J.Y., Huang Y.C., Huang W.H., Xia X.Z., Yang X.H., and Mou H.F., 2018, High-throughput identification and marker development of perfect SSR for cultivated genus of passion fruit (Passiflora edulis), 9(13): 92-96 (doi: 10.5376/mpb.2018.09.0013)

Abstract

Simple sequence repeat (SSR) markers are characterized by high polymorphism, good reproducibility and co-dominance etc. They can be easily applied to develop efficient, simple and practical molecular markers. In the present study, bioinformatics methods were applied to identify high-throughput perfect SSRs of cultivar Passiflora genome. A total of 13,104 perfect SSRs were obtained. SSR core sequence structure is mainly 2-4 bases, the maximum numbers are TA, AT, TC and AG. The maximum numbers of repetitions were up to 20 times. A total of 12,934 pairs of SSR markers were developed by using bioinformatics software, and 20 pairs of markers were selected for amplification specificity assessment of MTX and WJ10, and the polymorphism rate was as high as 60%. The large-scale development of the SSR markers of Passiflora cultivar has paved a foundation for the efficient utilization of the germplasm resources of passion fruit, genetic improvement of the varieties and molecular breeding.

Keywords
Passion fruit (Passiflora edulis); Genome; Perfect SSR; High-throughput; Molecular marker
[Full-Text PDF] [Full-Flipping PDF] [Full-Text HTML]
Molecular Plant Breeding
• Volume 9
View Options
. PDF(344KB)
. FPDF
. HTML
. Online fPDF
Associated material
. Readers' comments
Other articles by authors
pornliz suckporn porndick pornstereo . yanyan Wu
. Qinglan Tian
. Jieyun Liu
. Yongcai Huang
. Weihua Huang
. Xiuzhong Xia
. Xinghai Yang
. Haifei Mou
Related articles
. Passion fruit ( Passiflora edulis )
. Genome
. Perfect SSR
. High-throughput
. Molecular marker
Tools
. Email to a friend
. Post a comment