Research Article

Analysis of Key Enzyme Genes in Carotenoid Metabolism Pathway of Lilium and Cloning of LoLcyB  

Jing Liang1 , Li Wang1 , Rong Ding1 , Jinteng Cui1,2,3 , Kezhong Zhang1,2,3,4
1 Landscape Architecture School, Beijing University of Agriculture, Beijing, 102206, China
2 Beijing Laboratory of Urban and Rural Ecological Environment, Beijing, 102206, China
3 Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Tree, Beijing, 102206, China
4 Beijing Engineering Research Center of Rural Landscape Planning and Design, Beijing, 102206, China
Author    Correspondence author
Molecular Plant Breeding, 2019, Vol. 10, No. 4   doi: 10.5376/mpb.2019.10.0004
Received: 02 Jan., 2019    Accepted: 18 Jan., 2019    Published: 25 Jan., 2019
© 2019 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2018, 16: 4520-4529) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Liang J., Wang L., Ding R., Cui J.T., and Zhang K.Z., 2019, Analysis of key enzyme genes in carotenoid metabolism pathway of Lilium and cloning of LoLcyB, Molecular Plant Breeding, 10(4): 23-33 (doi: 10.5376/mpb.2019.10.0004)

Abstract

In this study, five growth stages of Oriental lily ‘Justina’ leaves (10 d, 25 d, 50 d, 75 d and 100 d), and seven different parts of styles, anthers, petals, leaves, stems, bulbs and roots in 100 day growth were used as test materials to measure the content of carotenoid and chlorophyll in Lilium by organic solvent method. The semi-quantitative expression analysis of 12 key enzyme genes (PSY, PDS, Z-ISO, ZDS, CRTISO, LCYB, LCYE, HYB, VDE, ZEP, CCS and NCED) of Lilium carotenoid metabolic pathway was carried out according to the sequencing results of transcriptome library. The study cloned the open reading frame (ORF) sequence of Lilium LCYB gene and carried out related bioinformatics analysis. The results showed that carotenoid and chlorophyll content were significantly different in the five different stages of Lilium leaf growth and seven different parts of Lilium ripening stage. The expression levels of 12 key enzyme genes were variant in different growth stages and parts. The complete ORF of the LCYB gene was cloned with the whole length of 1,503 bp, encoding 500 amino acids, which had typical conserved region characteristics of Carotene Cycl super family. It showed the closest relationship with Musa acuminata among the known genome-wide plants. This study might provide a solid theoretical basis for further study of Lilium carotenoid metabolic pathways and further understanding of the molecular mechanism of Lilium color and leaf color synthesis.

Keywords
Lilium brownii; Carotenoid metabolic pathway; LoLcyB; Semi-quantitative PCR
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