Molecular Tagging Wheat Powdery Mildew Resistance Gene Pm21 by EST-SSR and STS Markers
1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, P.R. China
2.Taiâ€™an Subcenter of National Wheat Improvement Center, Agronomy College of Shandong Agricultural University, Tai'an, 271018, P.R. China
Molecular Plant Breeding, 2010, Vol. 1, No. 4 doi: 10.5376/mpb.2010.01.0004
Received: 06 May, 2010 Accepted: 10 Jul., 2010 Published: 28 Sep., 2010
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Preferred citation for this article:
Qi et al., 2010, Molecular tagging wheat powdery mildew resistance gene Pm21 by EST-SSR and STS markers, Molecular Plant Breeding Vol.1 No.4 (doi: 10.5376/mpb.2010.01.0004)
In order to enrich the molecular markers linked to wheat powdery mildew resistance gene Pm21 and facilitate the molecular marker-assisted selection in multi-background breeding procedure, preferred small groups (PSG) analysis was conducted using F2 segregating population cross between the wheat germplasm line CB033 with Pm21 and a common wheat variety Huixianhong (susceptible to wheat powdery mildew). One hundred and two markers revealed polymorphism between the parents when screening with 258 SSR or STS markers located on wheat homoeologous group VI, and two EST-SSR markers (Xcfe164 and Xedm129) and one STS marker (Xcinau188) showed good consistency between the genotype and the phenotype to mildew in PSG. Linkage relationship between Pm21 and molecular markers of Xcfe164, Xedm129 and Xcinau188 were then established, with the distance 3.75 cM, 1.26 cM and 0.98 cM respectively. Authenticity verification of the linkage between Pm21 and molecular markers were tested via F2: 3 families and results showed that the three markers were reliable new markers linked to Pm21. The results above would facilitate molecular marker-assisted selection in breeding program.
Pm21; Preferred small group (PSG); Molecular tagging; EST-SSR; STS