Mutagenic Effects of Maize Inbred Line Irradiated by Heavy Ion 7Li
School of Sciences, Hebei University of Technology,Tianjin 300401, P.R.China
Molecular Plant Breeding, 2012, Vol. 3, No. 13 doi: 10.5376/mpb.2012.03.0013
Received: 18 Sep., 2012 Accepted: 20 Sep., 2012 Published: 31 Oct., 2012
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Preferred citation for this article:
Geng et al., 2012, Mutagenic Effects of Maize Inbred Line Irradiated by Heavy Ion 7Li, Molecular Plant Breeding, Vol.3, No.13 132-138 (doi: 10.5376/ mpb.2012.03.0013)
The dry seeds of maize inbred line Yong 19 were radiated by 7Li heavy ion with the energy 43MeV and the doses were treated with 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy, respectively. The field data for plant height and ear position of maize inbred line induced by heavy ion from M1 (mutation generation 1) to M3 were assessed. The results showed that the plant height of the offsprings derived from maize inbred line Yong 19 had significant variation by comparing with that of the control (CK). Genetic fingerprints of M4 progenies were analyzed by using random amplified polymorphic DNAs (RAPD) method, the results revealed that there were different effects on maize genome DNA by different doses. The doses of 10 Gy and 40 Gy could yield up higher optimal selective coefficiencies that were about 0.221 4 and 0.166 4, respectively. Compared with the Control, the genetic similarity (GS) coefficients of the doses in 10 Gy and 40 Gy were less than that of other doses. The maize radiated of 10 Gy and 40 Gy were classified into two different groups, and those of 20 Gy, 30 Gy, 50 Gy were congregated into another group.
Maize inbred line; Heavy ion; Dose; RAPD; Genetic similarity