A cDNA-AFLP Based Linkage Map Constructed with 67 Loci in F2 Population of Caixin (Brassica rapa var. parachinensis)  

Weidong Shi , Wenjia Li , Hailing Luo , Hongwei Kang , Jianhui Zhou , Chuifang Yang
The Institute of Vegetable Guangxi Academy of Agricultural Sciences, Nanning, 530007, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2012, Vol. 3, No. 14   doi: 10.5376/mpb.2012.03.0014
Received: 02 Nov., 2012    Accepted: 12 Nov., 2012    Published: 15 Nov., 2012
© 2012 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Shi et al., 2012, A cDNA-AFLP Based Linkage Map Constructed with 67 Loci in F2 Population of Caixin(Brassica rapa var. parachinensis), Molecular Plant Breeding, Vol.3, No.14 139-143 (doi: 10.5376/ mpb.2012.03.0014)

Abstract

A cDNA-AFLP based linkage map of Caixin (Brassica rapa var. parachinensis) was constructed by an F2 population with 92 individuals from a cross of the parents of early flowering and late flowering Caixin. The fifteen selective primers generated total 229 bands with the average of 15.3 bands per primer 113 bands were polymorphic accounting for 17.5 bands per primer. The high banding primer combination was ME1M1 yielding 30 bands while the low banding primer combination was E2M5 only yielding 8 bands. The primer combination, E3M1, generated the most polymorphic bands (120), but the E1M4 and E2M5 were the least (4). Chi square test (χ2) showed there was significant segregation distortion existing in the population. The linkage map was constructed based on cDNA-AFLP data, which consists of 4 linkage groups including 67 loci and spanning 334 cM with an average genetic distance of 4.99 cM.

Keywords
Caixin (Brassica rapa var. parachinensis); cDNA-AFLP; Molecular linkage map
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