Genetic Diversity Studies in Ragi [Eleusine coracana (L.) Gaertn.] With SSR and ISSR Mrkers
N. Meenakshi ganesan
Department of Botany Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore 641003, India
Molecular Plant Breeding, 2013, Vol. 4, No. 17 doi: 10.5376/mpb.2013.04.0017
Received: 27 May, 2013 Accepted: 31 May, 2013 Published: 31 May, 2013
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Preferred citation for this article:
Prabhu et al., 2013, Genetic Diversity Studies in Ragi (Eleusine coracana (L.) Gaertn.) With SSR and ISSR Mrkers, Molecular Plant Breeding, Vol.4, No.17 141-145 (doi: 10.5376/mpb.2013.04.0017)
Investigation was carried out on a collection of forty ragi genotypes to study the genetic diversity using ten SSR and 30 ISSR markers. There were successful amplification SSR and ISSR markers in ragi. Of the ten SSR primers six showed polymorphism, the number of alleles produced by SSR primers ranged from two to five with an average of 2.3 allele per primer with total of 23 alleles. The percentage of amplification ranged from 58 (RM 570) to 92 (RM 3351). Of the thirty ISSR primers used for molecular analysis, twenty primers showed polymorphism among forty ragi genotypes. The twenty polymorphic primers generated a total of 101 alleles. The number of alleles produced by different primers ranged between three and ten with an average of 5.05 alleles per primer. The percentage of amplification ranged from 33 (ISSR 20) to 85 (ISSR 29). Forty genotypes grouped into thirteen clusters (SSR) and five clusters (ISSR), respectively in UPGMA analysis.
Ragi; SSR marker; ISSR marker; Genetic diversity; UPGMA analysis