Optimization of in vitro and ex vitro Regeneration in Sarcostemma Brunonianum Wight & Arn. – An Endemic Climber
1 Biotechnology Laboratory, Department of Plant Science, M.G.G.A.C., Mahe, Pondicherry, India
2 Department of Botany, K.M. Centre for Postgraduate Studies, Pondicherry, India
Medicinal Plant Research, 2016, Vol. 6, No. 5
Received: 22 Mar., 2016 Accepted: 03 Apr., 2016 Published: 21 Jul., 2006
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The present report describes an in vitro propagation protocol and regeneration of adventitious roots from stem derived callus in Sarcostemma brunonianum, an endemic medicinal plant. The effect of cytokinins, 6-benzylaminopurine (BAP) and kinetin (Kin) at different concentrations on in vitro shoot multiplication from nodal explants of S. brunonianum was evaluated. Bud breaking and emergence of shoots was observed within 2 week on Murashige and Skoog (MS) medium containing 1.5 mg/L BAP, resulted in maximum number of shoots (4.0±1.0) from nodal explants. Maximum 22.7 shoots were multiplied on MS medium + 0.5 mg/L each of BAP and Kin. In vitro raised shoots were rooted on half strength MS medium supplemented with 1.0 mg/L indole-3-butyric acid (IBA). Maximum roots were induced by the ex vitro methods from the cut ends of the shoots with 300 mg/L IBA. This is the first report on in vitro propagation and ex vitro rooting of Sarcostemma brunonianum. The rooted plantlets were hardened in the greenhouse for another two months and finally shifted to the soil under natural conditions with 86% rate of survival The in vitro regenerated shoots were used to regenerate callus on MS medium containing 2,4-D (2,4-Dichlorophenoxyacetic acid). The adventitious roots were induced and proliferated from the callus tissues on half strength semi-solid MS medium augmented with 1.0 mg/L IBA. The maximum fresh weight (0.875 gm) and dry biomass (0.0720 gm) of roots was reported with 1.0 mg/L IBA.
Sarcostemma brunionum; Endemic; Ex vitro rooting; Callus; Adventitious roots
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