Expression Analysis of Puccinellia tenuifolra Gene PutSTE24 and Response to Aluminium Stresses  

Baoxing Wang1 , Bo Sun1 , Xinxin Zhang1 , Shenkui Liu1 , Tetsuo Takano2 , Yuanyuan Bu1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China
2. Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2014, Vol. 5, No. 2   doi: 10.5376/msb.2014.05.0002
Received: 16 Jan., 2014    Accepted: 20 Feb., 2014    Published: 25 Apr., 2014
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

 Bu et al., 2014, Expression Analysis of Puccinellia tenuifolra Gene PutSTE24 and Response to Aluminium Stresses, Molecular Soil Biology, Vol.5, No.2 9-15 (doi: 10.5376/msb.2014.05.0002)

Abstract

Alkaline grass (Puccinellia tenuiflora) can serve as a model of salt tolerance in monocotyledon crops. However, little is known about its mechanism of aluminum stress. In this work, we screened PutSTE24 gene under aluminum stress to predict its subcellular localization of proteins by using bioinformatics analysis software, and the result showed that this gene was in the endoplasmic reticulum, which was the same with expressing PutSTE24 gene in yeast INVsI. Under aluminum and saline-alkali stress conditions, the relative mRNA expression levels of the PutSTE24 of alkaline grass were measured by real-time quantitative PCR, and the results demonstrated that its expression was induced up regulation. We also constructed a plant expression vector of PutSTE24 gene, and then transferred into Arabidopsis. For further determine the correlation between PutSTE24 gene and aluminum stress, we choose transgenic seeds for resistance experiment, which provided a wealth of theoretical and experimental basis for further research on interaction of related genes and regulatory mechanism of aluminum stress.

Keywords
Puccinellia tenuifolra; PutSTE24 gene; Aluminum stresses; Transgenic Arabidopsis
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