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Isolation and Resistance Analysis of Algae of Alkali Spot Soil | Wang 1 | Molecular Soil Biology

Isolation and Resistance Analysis of Algae of Alkali Spot Soil  

Juan Wang1 , Shi Wei1 , Takano Tetsuo2 , Shenkui Liu1,2
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, P.R. China;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2012, Vol. 3, No. 1   doi: 10.5376/msb.2011.03.0001
Received: 08 Jun., 2011    Accepted: 20 Jun., 2011    Published: 28 Jun., 2011
© 2012 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang et al., 2011, Isolation and Resistance Analysis of Algae of Alkali Spot Soil, Molecular Soil Biology, doi:10.5376/msb.2011.03.0001

Abstract

Algae can survive in a very wide range environment and have high adaptability for a variety of adversity. In present study, we selected alkali spot soil in Anda City, Heilongjiang Province as the algae research plots. In addition, we selected garden nursery soil in Northeast Forestry University as the control. With streak plating method, we isolated 30 algae species eventually, 29 of which had been purified. After resistance screening of 29 algae species by NaCl stress and NaHCO3 stress, we found their growth conditions were different from each other at different stress or different stress concentration gradient. Algae species of soda soil showed stronger resistance than those of nursery soil. Moreover, some algae were strongly inhibited by lower concentration of sodium bicarbonate, but some were promoted.

Keywords
Alkali spot soil; Soil algae; Salt-stress; Isolation and identification; Resistance
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