Cloning and Characterization of PutSTE24 Gene from Puccinellia tenuifolra Which Expressed in Response to Abiotic Stresses
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, P.R. China;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Molecular Soil Biology, 2011, Vol. 2, No. 2 doi: 10.5376/msb.2011.02.0002
Received: 03 Jun., 2011 Accepted: 20 Jun., 2011 Published: 19 Jun., 2011
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Preferred citation for this article:
Zhang et al., 2011, Cloning and Characterization of PutSTE24 Gene from Puccinellia tenuifolra Which Expressed in Response to Abiotic Stresses, Molecular Soil Biology, doi:10.5376/msb.2011.02.0002
The Puccinellia tenuifolra cDNA libraries were expressed in yeast (Saccharomyces cerevisiae) and screened on agar plates containing toxic concentrations of aluminum. Ninteen cDNAs were isolated that enhanced the aluminum tolerance of yeast. One cDNA, named PutSTE24, has a ORF of 1 275 bp, encoding a predicted protein containing 424 amino acid, and has a high similarity of 77% to STE24 in Arabidopsis thilinana. PutSTE24 and AtSTE24 were transformed into yeast cells separately and were treated with AlCl3, salt, drought, low pH and oxidation and metal ions stresses. Results revealed that these two recombinant yeast cells showed similarly and grew better in AlCl3, salt and oxidation stresses than control cells, but no obvious differences in low pH and drought stresses. Additionally, on the responsive to the metal ions, these two genes have obvious resistance to the stresses of K+, Mg2+ and Cu2+, are somewhat resistant to Fe3+,Cd2+, and have no obvious responsive relationship with Ca2+, Mn2+ and Ba2+, but to the metal ions of Co2+, Ni2+and Zn2+, these two recombinant yeast cells are sensitive, growing worse than the control cells, especially the Zn2+. It is basically confirmed the gene STE24 is related to metal stresses, which has no report in the previous studies.
Puccinellia tenuifolra; PutSTE24 gene; Yeast; Stresses