Isolation and Characterization of An Alkaliphilic and Halotolerant Nesterenkonia.sp from An Extreme Soda Saline-alkali Soil in the Northeastern of China  

Wei Shi1 , Takano Tetsuo2 , Shenkui Liu1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education,; Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, 150040;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2012, Vol. 3, No. 2   doi: 10.5376/msb.2011.03.0002
Received: 29 Jun., 2011    Accepted: 01 Aug., 2011    Published: 09 Aug., 2011
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Shi et al., 2011, solation and characterization of an alkaliphilic and halotolerant Nesterenkonia.sp from an extreme soda saline-alkali soil in the northeastern of China, Molecular Soil Biology, doi:10.5376/msb.2011.03.0002

Abstract

A strain of alkaliphilic and halotolerant, rod-shaped bacterium, designated strain SA-3, was solated from an Extreme soda saline-alkali soil in Heilongjiang, northeastern China. Strain SA-3 was Gram-positive, catalase-positive, and oxidase-negative and formed Orange colonies, and starch is not hydrolyzed, optimal growth conditions were pH 9.0 and 0.5 mol/L NaCl. Strain SA-3 has strong resistance to Na2CO3, hight pH value, and NaCl. 16S rRNA a gene sequence alignment analysis show that the strain formed a separated line within a clade containing the genus Nesterenkonia in the phylum Actinobacteria and was related to the species Nesterenkonia lutea YIM 70081T (sequence similarity 99.2%), Nesterenkonia sandarakina YIM 70009T (99.2%), Nesterenkonia jeotgali JG-241T (99.1%), Nesterenkonia halotolerans YIM 70084T (99.0%), Nesterenkonia aethiopica DSM 17733T (97.5%). This similarity, suggest that this salt-tolerant alkaliphilic bacterium belonged to genus Nesterenkonia. This strain is a great potential microbial resources for development.

Keywords
Nesterenkonia; 16S rDNA sequence
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