Isolation and Characterization of An Alkaliphilic and Halotolerant Nesterenkonia.sp from An Extreme Soda Saline-alkali Soil in the Northeastern of China
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education,; Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, 150040;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Molecular Soil Biology, 2012, Vol. 3, No. 2 doi: 10.5376/msb.2011.03.0002
Received: 29 Jun., 2011 Accepted: 01 Aug., 2011 Published: 09 Aug., 2011
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Preferred citation for this article:
Shi et al., 2011, solation and characterization of an alkaliphilic and halotolerant Nesterenkonia.sp from an extreme soda saline-alkali soil in the northeastern of China, Molecular Soil Biology, doi:10.5376/msb.2011.03.0002
A strain of alkaliphilic and halotolerant, rod-shaped bacterium, designated strain SA-3, was solated from an Extreme soda saline-alkali soil in Heilongjiang, northeastern China. Strain SA-3 was Gram-positive, catalase-positive, and oxidase-negative and formed Orange colonies, and starch is not hydrolyzed, optimal growth conditions were pH 9.0 and 0.5 mol/L NaCl. Strain SA-3 has strong resistance to Na2CO3, hight pH value, and NaCl. 16S rRNA a gene sequence alignment analysis show that the strain formed a separated line within a clade containing the genus Nesterenkonia in the phylum Actinobacteria and was related to the species Nesterenkonia lutea YIM 70081T (sequence similarity 99.2%), Nesterenkonia sandarakina YIM 70009T (99.2%), Nesterenkonia jeotgali JG-241T (99.1%), Nesterenkonia halotolerans YIM 70084T (99.0%), Nesterenkonia aethiopica DSM 17733T (97.5%). This similarity, suggest that this salt-tolerant alkaliphilic bacterium belonged to genus Nesterenkonia. This strain is a great potential microbial resources for development.
Nesterenkonia; 16S rDNA sequence