Identification and characterization of a cation2+/H+ antiporter AtCAX4 gene from Arabidopsis thaliana
1 Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
2 Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, Japan
Molecular Soil Biology, 2016, Vol. 7, No. 5 doi: 10.5376/msb.2016.07.0005
Received: 05 May, 2016 Accepted: 01 Jun., 2016 Published: 07 Jun., 2016
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Preferred citation for this article:
Jiangpo Chen, Testuo Takano, Shenkui Liu, and Yuanyuan Bu, 2016, Identification and Characterization of a Cation2+/H+ Antiporter AtCAX4 Gene from Arabidopsis thaliana, Molecular Soil Biology, 7(5): 1-5 (doi: 10.5376/msb.2016.07.0005)
AtCAX4 is a member of the Ca2+/H+ exchangers (CAX) family was cloned from cDNA library of Arabidopsis thaliana. AtCAX4 was expressed in all Arabidopsis plant organs, with the highest level in root under normal conditions by quantitative real-time PCR analysis. The expression of AtCAX4 gene is response to Na+, Ca2+ and Ba2+ in both the leaves and roots of Arabidopsis. In addition, the pYES2-AtCAX4 transformants confers tolerance to Na+, Ca2+ and Ba2+ ions in yeast. These results suggest that AtCAX4 play important role in increasing the Na+, Ca2+ or Ba2+ tolerance of yeast.
AtCAX4; Gene expression; Tolerance; Yeast