Research Article

Identification and characterization of a cation2+/H+ antiporter AtCAX4 gene from Arabidopsis thaliana  

Jiangpo Chen1 , Testuo Takano2 , Shenkui Liu1 , Yuanyuan Bu1
1 Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
2 Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2016, Vol. 7, No. 5   doi: 10.5376/msb.2016.07.0005
Received: 05 May, 2016    Accepted: 01 Jun., 2016    Published: 07 Jun., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Jiangpo Chen, Testuo Takano, Shenkui Liu, and Yuanyuan Bu, 2016, Identification and Characterization of a Cation2+/H+ Antiporter AtCAX4 Gene from Arabidopsis thaliana, Molecular Soil Biology, 7(5): 1-5 (doi: 10.5376/msb.2016.07.0005)

Abstract

AtCAX4 is a member of the Ca2+/H+ exchangers (CAX) family was cloned from cDNA library of Arabidopsis thaliana. AtCAX4 was expressed in all Arabidopsis plant organs, with the highest level in root under normal conditions by quantitative real-time PCR analysis. The expression of AtCAX4 gene is response to Na+, Ca2+ and Ba2+ in both the leaves and roots of Arabidopsis. In addition, the pYES2-AtCAX4 transformants confers tolerance to Na+, Ca2+ and Ba2+ ions in yeast. These results suggest that AtCAX4 play important role in increasing the Na+, Ca2+ or Ba2+ tolerance of yeast.

Keywords
AtCAX4; Gene expression; Tolerance; Yeast
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