Molecular cloning, expression, and purification of a urease accessory protein UreG of Arabidopsis thaliana
1 Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
2 Asian Natural Environmental Science Center (ANESC), the University of Tokyo, Tokyo 188-0002, Japan
Molecular Soil Biology, 2016, Vol. 7, No. 6 doi: 10.5376/msb.2016.07.0006
Received: 05 May, 2016 Accepted: 01 Jun., 2016 Published: 08 Jun., 2016
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Preferred citation for this article:
Kou J., Takano T., Liu S., and Bu Y.Y., 2016, Molecular Cloning, Expression, and Purification of a Urease Accessory Protein UreG of Arabidopsis thaliana, Molecular Soil Biology, 7(6): 1-6 (doi: 10.5376/msb.2016.07.0006)
UreG is one of the accessory proteins of urease in plants. In this study, a gene encoding an UreG consisting of 363 amino acids from Arabidopsis thaliana was isolated. The AtUreG gene was expressed in Escherichia coli BL21 as a GST fusion protein. The GST-AtUreG fusion protein was induced and purified under the optimized culturing conditions of 0.2 mM IPTG and 25℃ for 5 h. Western blot analysis using an anti-GST antibody showed that the GST-AtUreG fusion protein was not degraded.
AtUreG; Protein expression; Arabidopsis thaliana