Function Analysis of cDNA Clone Genes from Puccinellia tenuiflora under NaHCO3 Stress
James X.J. Fang1,2
1. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, No. 26 Hexing Road, Nangang District, Harbin 150040, China; Laboratory of Soybean Molecular Biology and Molecular Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, No. 138 Haping Road, Nangang District, Harbin 150081, China
2. Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, China
Molecular Soil Biology, 2016, Vol. 7, No. 9 doi: 10.5376/msb.2016.07.0009
Received: 25 Mar., 2016 Accepted: 12 Apr., 2016 Published: 12 Apr., 2016
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Preferred citation for this article:
Liu P.P., Sun Y.J., Ye X.X., and Fang J.X.J., 2016, Function Analysis of cDNA Clone Genes from Puccinellia tenuiflora under NaHCO3 Stress, Molecular Soil Biology, 7(9): 1-16 (doi: 10.5376/msb.2016.07.0009)
Puccinellia tenuiflora is widely found in saline-alkali soil of the Songnen plain in northeastern China, which indicates it has a high tolerance to saline-alkali stress. In this study, we selected 3,072 clones from cDNA library of P. tenuiflora with NaHCO3 treatment. In the end, 2991 clones were chosen for subsequent analysis. We use blastx to explore the homology relationship between P. tenuiflora and other plants. Among 2991 clones, approximately 89% were annotated in more than one public protein database and nearly 25% of the clones showed significant similarities with Hordeum vulgare. The clone sequences are classified in carbohydrate metabolism, photosystem, response to stimulus, ribosome, defense mechanism, metal/ion binding and hormone-mediate signal, etc. And from the 2991 clones, we identified 184 clones which may be involved in salt stress by search pubmed database with corresponding keywords. This study offers new molecular functions and mechanisms of P. tenuiflora and may also provide valuable reference for exploring potential molecular mechanisms of saline-alkali tolerance in P. tenuiflora.
Puccinellia tenuiflora; NaHCO3 stress; cDNA clone; Function annotation