Analysis of the Molecular Marker Related to Early-Bearing in Maternal Parent and F1 Generation of Walnut by SCAR
1. Department of Horticulture, Agricultural College of Shihezi University, Shihezi, 832003, P.R. China
2. Center for Molecular Agrobiotechnology and Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, P.R. China
Plant Gene and Trait, 2012, Vol. 3, No. 4 doi: 10.5376/pgt.2012.03.0004
Received: 14 Dec., 2011 Accepted: 12 Jan., 2012 Published: 13 Jan., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Ye et al., 2012, Analysis of the Molecular Marker Related to Early-Bearing in Maternal Parent and F1 Generation of Walnut by SCAR, Plant Gene and Trait, Vol.3, No.4 18-21 (doi: 10.5376/pgt.2012.03.0004)
In this study, we analyzed the heredity rule of SCAR molecular marker related to early-bearing trait in maternal parents and F1 generations of walnut in Xinjiang. By PCR amplification and sequencing, the SCAR molecular marker related to early-bearing trait in walnut was obtained, which was 762 bp. PCR amplification of the maternal parents and their F1 generations was carried out with the specific primers, the results showed that the fragment of SCAR molecular marker about 762 bp appeared in the four maternal parents tested material. And the percentage of F1 generations in all cultivars containing this marker was 92.68%, 97.92%, 96.43%, 93.10% and 97.73%, respectively, which indicated that the early-bearing trait was high heredity ability in walnut. This study would give us some insights into studying gene cloning and its function in early-bearing walnut of Xinjiang Province in future.
Walnut; Early-bearing; SCAR; F1 generations