Development of a New japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, 210014
Rice Genomics and Genetics, 2010, Vol. 1, No. 3 doi: 10.5376/rgg.2010.01.0003
Received: 22 Oct., 2010 Accepted: 21 Nov., 2010 Published: 25 Dec., 2010
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Preferred citation for this article:
Wang et al., 2009, Development of a New japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection, Molecular Plant Breeding, 7(6): 1070-1076
In order to developing a new japonica rice variety with both good eating quality and high yield, we employed the high yield japonica rice "Wu-xiang-jing 14" as the maternal parent was crossed to the good quality japonica rice "Kantou 194" with low-amylose content and translucent endosperm controlled by Wx-mq. By comparing the DNA sequence of Wx-mq gene and its allelic genes Wx-b, Wx-a, wx in chromosome 6, two mutated bases occurring in exon 4 and exon 5 were found in Wx-mq. The change of G-A in exon 4 just produced a recognition site for NIaâ…¢ digestion, so a cleaved amplified polymorphic sequences (CAPS) marker was designed, which could identify the Wx-mq homozygous genotype and heterozygous genotype. This marker was used for marker assisted selection (MAS) of low-amylose content in F5 and F6 lines derived from the combination of Wu-xiang-jing 14/Kantou 194. The investigation result of endosperm appearance was completely coincident with that of molecular detection, and the efficiency of MAS with the CAPS marker was up to 100%. As a result, “Nan-jing 46”, a new japonica rice variety with good eating quality and high yield, was successfully developed.
Marker assisted selection; Good eating quality; Rice; Cleaved amplified polymorphic sequences (CAPS)