Development of a New japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection  

Cailin Wang , Yadong Zhang , Zhen Zhu , Tao Chen , Ling Zhao , Jing Lin , Lihui Zhou
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, 210014
Author    Correspondence author
Rice Genomics and Genetics, 2010, Vol. 1, No. 3   doi: 10.5376/rgg.2010.01.0003
Received: 22 Oct., 2010    Accepted: 21 Nov., 2010    Published: 25 Dec., 2010
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This article was first published in the Molecular Plant Breeding (Regular Print Version), and here was authorized to redistribute under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang et al., 2009, Development of a New japonica Rice Variety Nan-jing 46 with Good Eating Quality by Marker Assisted Selection, Molecular Plant Breeding, 7(6): 1070-1076

Abstract

In order to developing a new japonica rice variety with both good eating quality and high yield, we employed the high yield japonica rice "Wu-xiang-jing 14" as the maternal parent was crossed to the good quality japonica rice "Kantou 194" with low-amylose content and translucent endosperm controlled by Wx-mq. By comparing the DNA sequence of Wx-mq gene and its allelic genes Wx-b, Wx-a, wx in chromosome 6,  two mutated bases occurring in exon 4 and exon 5 were found in Wx-mq. The change of G-A in exon 4 just produced a recognition site for NIaâ…¢ digestion, so a cleaved amplified polymorphic sequences (CAPS) marker was designed, which could identify the Wx-mq homozygous genotype and heterozygous genotype. This marker was used for marker assisted selection (MAS) of low-amylose content in F5 and F6 lines derived from the combination of Wu-xiang-jing 14/Kantou 194. The investigation result of endosperm appearance was completely coincident with that of molecular detection, and the efficiency of MAS with the CAPS marker was up to 100%. As a result, “Nan-jing 46”, a new japonica rice variety with good eating quality and high yield, was successfully developed.

Keywords
Marker assisted selection; Good eating quality; Rice; Cleaved amplified polymorphic sequences (CAPS)
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