Genetic Diversity Study of Seed Proteins in Rice Drought Tolerant Donor Accessions
Crop Improvement Division, Central Rice Research Institute, Cuttack, Orissa, India
Rice Genomics and Genetics, 2015, Vol. 6, No. 2 doi: 10.5376/rgg.2015.06.0002
Received: 04 Feb., 2015 Accepted: 23 Mar., 2015 Published: 27 Mar., 2015
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Preferred citation for this article:
Haque et al., 2015, Genetic diversity study of seed proteins in rice drought tolerant donor accessions, Rice Genomics and Genetics, Vol.6, No.2 1-5 (doi: 10.5376/rgg.2015.06.0002)
Twenty four rice genotypes were analyzed under drought stress for seed proteins by SDS-PAGE to estimate their genetic diversity for the purpose of genetic improving under drought condition. The storage protein profiling banding pattern was scored based on the number and intensity of bands.A total of 90 poly peptide bands were harvested from twenty four individuals ranging from 14.3 kD to 99.0 kD. According to UPGMA dendrogram, 100% genetic similarities of protein profiling were exhibited between Basmati 370, Mahulata, Naveen and CR 143-2-2. The banding profile revealed that studied genotypes varies from each other either for total number or molecular weight or intensity of protein bands except the genotypes Naveen and CR 143-2-2. This electrophoretically detectable protein polymorphism in rice grain can be used in further breeding proposes and variety development. Our results suggest that, screening of these genotypes on the basis of variation in seed protein profile using SDS-PAGE could be highly effective tool to identify drought tolerant donors with good seed storage under drought stress.
Genetic diversity; Drought stress; Seed protein; Electrophoresis
Rice Genomics and Genetics
• Volume 6