Research Report

Introgressing Blast Resistant Gene Pi-9(t) into Elite Rice Restorer Luhui17 by Marker-Assisted Selection  

Shaoshan Wen1,2 , Bijun Gao1,2
1. Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Luzhou, 646100, P.R. China
2. Luzhou Branch of National Rice Improvement center, Luzhou, 646100, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2011, Vol. 2, No. 4   doi: 10.5376/rgg.2011.02.0004
Received: 31 Oct., 2011    Accepted: 07 Nov., 2011    Published: 16 Jan., 2012
© 2011 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wen et al., 2011, Introgressing Blast Resistant Gene Pi-9(t) into Elite Rice Restorer Luhui17 by Marker-Assisted Selection, Rice Genomics and Genetics, Vol.2, No.4 31-36 (doi: 10.5376/rgg.2011.02.0004)

Abstract

In this research, we introgressed the broad-spectrum blast resistant gene Pi-9(t) from the donor parent P2 into hybrid restorer Luhui17 by using backcross approach and molecular marker-assisted selection (MAS) technique. We detected the target gene through the specific molecular marker pB8 that close linked to the blast resistant gene Pi-9(t). As a result, sixty eight lines carrying broad-spectrum blast resistant gene Pi-9(t) were obtained, and then four lines, WR1023, WR1043, WR1056 and WR1062, were identified to be homozygous backcross lines with resistant gene Pi-9(t) by molecular phenotyping identification. Furthermore, the lines, WR1023 and WR1056 lines, were proved to have genetically stable agronomic characteristics, that demonstrated good plant architecture, high blast resistance and stable restoring ability, Therefore we suggested that these lines should be used as resistant gernplasms in in rice hybrid breeding program.

Keywords
Rice (Oryza sativa L.); Blast resistance; Pi-9(t); Molecular marker-assisted selection; Rice restorer
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