Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS System  

Xianju Wang1,2 , Xuelin Tan1 , Guojun Shao2 , Hui Ma3 , Yajun Chen2 , Cheng Zhang2 , Wenqi Shang2
1. Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R. China;
2. Rice Research Institute, Liaoning Province, Shenyang, 110101, P.R. China;
3. Shenyang Agricultural University, Shenyang, 110161, P.R. China
Author    Correspondence author
Rice Genomics and Genetics, 2012, Vol. 3, No. 4   doi: 10.5376/rgg.2012.03.0004
Received: 15 Mar., 2012    Accepted: 28 Apr., 2012    Published: 02 May, 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (Vol.8, No.2, 319-324) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang et al., 2012, Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS system, Rice Genomics and Genetics, Vol.3, No.4 19-24 (doi: 10.5376/rgg.2012.03.0004)

Abstract

In this paper, we employed the rice variety Wanlun 422 with wide compatibility, WA-type restorer line Shuhui 527, Miyang 46, and Miyang 42 as the parent materials, and selected the Japonica rice restorer line for Yinshui type cytoplasmic male sterility (YS-CMS) from the hybrid combinations F2 and F4 using molecular marker-assisted selection combined with the conventional breeding method. The results showed that the plants containing two restorer genes identified by molecular markers were selected to make hybrid combinations with YS-CMS lines. We found that the seed-setting rate and heterosis of the hybrids were significantly improved. The results of this paper indicate that it is feasible for development of restorers for Japonica YS-CMS line based on molecular marker-assisted.

Keywords
Yinshui type cytoplasmic male sterility (YS-CMS); Japonica restorer line; Marker-assisted selection; Gamete generation
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