Design and Validation of Two InDel Markers for Low Glutelin Content (Lgc1) Gene in Rice (Oryza sativa L.)
1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, 210014, P.R. China
2. Bijie Institute of Agricultural Science, Bijie, 551700, P.R. China
Rice Genomics and Genetics, 2012, Vol. 3, No. 8 doi: 10.5376/rgg.2012.03.0008
Received: 20 Aug., 2012 Accepted: 31 Aug., 2012 Published: 07 Sep., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2010, Vol.8, No.2, 340-344) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Tian et al., 2012, Design and Validation of Two InDel Markers for Low Glutelin Content (Lgc1) Gene in Rice (Oryza sativa L.), Vol.3, No.8 50-54 (doi: 10.5376/rgg.2012.03.0008)
Rice with low glutelin content is quite effective as a kind of functional food for patients with kidney disease. It is of great importance to develop commercial rice varieties of low glutelin content. In recent years, Lgc1 gene, as an excellent genetic resource for rice varieties with low glutelin content, has been given more and more attention by breeders in functional rice breeding. To improve the precision of marker-assisted selection for Lgc1 gene in rice, we designed two pairs of functional markers for Lgc1 gene based on a 3.5 kb deletion fragment between two highly similar glutelin genes GluB4 and GluB5 in two rice mutants. Two InDel markers were designated and named In- Del-Lgc1-A and InDel-Lgc1-B, respectively. These markers were used to analyze 13 rice varieties and the F2 segregation population derived from the cross of the low glutelin content rice variety W3660 and normal variety Nangjing46. The PCR results showed that according to the band types the homozygous genotype with low glutelin content, the heterozygous genotype, and the homozygous genotype with normal glutelin content could be clearly distinguished, which was completely consistent with the results of the protein analysis. Thus, these two InDel markers could be used to distinguish rice varieties with Lgc1 gene, and can also be used in marker-assisted selection.
Rice; Low glutelin; Lgc1 gene; InDel marker; Marker-assisted selection