Research Article

Fast Neutron Radiation Induced Glu-B1 Deficient Lines of an Elite Bread Wheat Variety  

Debbie Laudencia-Chingcuanco
Crop Improvement and Genetics Research Unit, USDA-ARS WRRC, 800 Buchanan Street, Albany, CA 94710, USA
Author    Correspondence author
Triticeae Genomics and Genetics, 2017, Vol. 8, No. 1   
Received: 28 Sep., 2016    Accepted: 01 Dec., 2016    Published: 12 Apr., 2017
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

Five isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded by the B-genome were identified from a fast-neutron radiation-mutagenized population of Summit, an elite variety of bread wheat (Triticum aestivum L.).  The mutant lines differ from the wild-type progenitor by the absence of the HMW-GS Bx17 and By18 proteins.  Gene specific primers designed for Bx17 and By18 genes failed to generate the diagnostic amplicons in the mutant lines. Assays using the iSelect Illumina Wheat 90K SNP arrays revealed that the induced deletions in these lines ranged from 5.58 to 127.47 cM.  Isogenic wheat lines deficient in the different HMW-GS proteins will allow the determination of the contributions of these different genes to dough quality. This new germplasm will serve as genetic tools to generate healthier wheat varieties with altered dough performance for diverse end-uses. 

Keywords
Wheat; Glutenin; HMW-GS; Glu-B1; Bread baking; Mutant; Bx17+By18; Fast-neutron

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Triticeae Genomics and Genetics
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